Advances in tissue optical clearing: prospects for in vivo applications
September 22nd, 2017 VALERY V. TUCHIN Saratov State University

This lecture presents fundamentals and advances of tissue optics and tissue optical clearing (OC). Controlling of tissue optical properties is based on using immersion technique via application of exogenous or endogenous optical clearing agents (OCAs). Impact of OCA and water transport in a tissue caused by an action of a hyperosmotic OCA and leading to tissue reversible shrinkage and dehydration on temporal tissue optical properties is discussed. The specific features of optical clearing of fibrous and cell-structured tissues are investigated using different linear and nonlinear optical modalities in the visible, NIR and terahertz ranges. In vitro, ex vivo, and in vivo studies of a variety of human and animal tissues, including skin, fat, eye sclera, muscle, cerebral membrane, cartilage, blood vessels, and blood are presented. The technologies of delivery of OCAs and nanoparticles are also under discussion, including hidden free diffusion, enforced tissue permeability, and via blood and lymph vessel networking. Examples of beneficial application of OC technology for enhancement of optical imaging modalities such as OCT, confocal, polarization-sensitive, Raman, two-photon excitation fluorescence microscopy, SHG-microscopy, photoacoustic imaging and cytometry, blood flow speckle imaging, and some others will be demonstrated.

Seminar, September 22, 2017, 10:00. Seminar Room

Hosted by Turgut Durduran