Development of quantitative, high spatiotemporal resolution imaging methods
Development of nano-tools for quantitative bio-imaging.
Super-resolution microscopy has revolutionized light microscopy, pushing the spatial resolution well below the diffraction limit. The impact of super-resolution methods have already been recognized by the Nobel Prize in Chemistry in 2014. Important challenges remain in pushing the application of these methods in biology. One of these limitations is the ability to extract quantitative information from the images, which is confounded by fluorophore photophysics. In addition, the temporal resolution is also limited by fluorophore photostability and photoswitching characteristics. We are developing methods to circumvent these limitations and extend the spatiotemporal resolution and quantitative power of super-resolution microscopy.
For more information see the BioNano@ICFO website.